独岛枝芽胞杆菌MCCC 1A00493产生的4-乙烯基苯酚鉴定和作用南方根结线虫特性研究

【目的】根结线虫危害严重,难防难控,前期研究发现一株深海来源的独岛枝芽胞杆菌(Virgibacillus dokdonensis) MCCC 1A00493对南方根结线虫具有良好的体外拮抗效果,本研究分离鉴定菌株发酵液中杀线虫活性物质,对其作用线虫的多种模式进行研究,为菌株有效控制植物病原线虫的应用奠定理论基础。【方法】采用硅胶柱层析和半制备高效液相色谱对独岛枝芽胞杆菌发酵上清液中的杀线虫活性物质进行分离纯化,采用液相色谱质谱联用、核磁共振对纯化物进行结构鉴定;并对其趋避、诱杀、熏杀和卵孵化抑制活性进行检测。【结果】结构鉴定确定独岛枝芽胞杆菌MCCC 1A00493发酵液中分离的杀线虫活性物质为4-乙烯基苯酚。4-乙烯基苯酚对线虫具有触杀、熏杀、卵孵化抑制和高浓度驱避低浓度引诱活性,15μg/mL 4-乙烯基苯酚72 h触杀线虫校正死亡率为71.23%±9.06%;20 mg/mL 4-乙烯基苯酚24 h熏杀线虫死亡率达100%;100μg/mL4-乙烯基苯酚作用线虫卵10d后,卵孵化抑制率达66.2%;在琼脂平板上,10mg/mL的4-乙烯基苯酚对线虫有驱避作用,1 mg/mL的4-...     

荒漠灌丛土壤动物分布及其生态功能

在干旱、半干旱荒漠生态系统中,灌丛作为一种重要的植被类型,其独特的形态和生理适应特性能够有效促进退化生态系统结构与功能的恢复。土壤动物是荒漠生态系统中不可或缺的重要组成部分,对促进灌丛"肥岛"演变具有重要的生态作用,有利于灌丛生态功能的发挥及退化生态系统的恢复。近年来,国内外学者对荒漠灌丛微生境土壤动物的研究逐步深入,取得大量的研究成果。在此基础上,首先综述荒漠灌丛微生境土壤动物群落分布和生态功能,总结灌丛与土壤动物分布间作用关系的数学模型,针对荒漠灌丛土壤动物研究中存在的问题提出了未来可能的研究方向和建议。     

Saldanha Bay,South Africa: recovery of Gracilaria verrucosa (Gracilariales,Rhodophyta)

Since World War II the greater Saldanha Bay lagoon system, South Africa, has been an important Gracilaria producer. Two agar factories, built in the 1960's, used Gracilaria from Saldanha Bay as their raw material. In the early 1970's the industry was destroyed as a result of dredging and marine construction operations to establish a harbor in the bay for loading ore. These environmental changes destroyed stocks and prevented the previously significant beachings of the seaweed from occurring. After a few years of no or very low commercial production, the resource slowly started to recover. The size of Gracilaria drifts increased over the following eight years to approximately one-third of the original output. This trend seems to continue. Although the stocks and resultant drifts are unlikely to recover fully to their original quantity, current production is already sufficient to ensure re-establishment of a seaweed industry in Saldanha Bay. This could have considerable socio-economic impact on the area.     

The energetics of middle-distance running

In order to assess the relative contribution of aerobic processes to running velocity (v), 27 male athletes were selected on the basis of their middle-distance performances over 800, 1500, 3000 or 5000 m, during the 1987 track season. To be selected for study, the average running velocity (v) corresponding to their performances had to be superior to 90% of the best French v of the season. Maximum O2 consumption (VO2max) and energy cost of running (C) had been measured within the 2 months preceding the track season, which, together with oxygen consumption at rest (VO2rest) allowed us to calculate the maximal v that could be sustained under aerobic conditions: vamax = (VO2max - VO2rest) x C-1. The treadmill running v corresponding to a blood lactate of 4 mmol.l-1 (vla4), was also calculated. In the whole group, C was significantly related to height (r = -0.43; P less than 0.03). Neither C nor VO2max (with, in this case, the exception of the 3000 m athletes) were correlated to v. On the other hand, vamax was significantly correlated to v over distances longer than 800 m. These v were also correlated to vla4. However vla4 occurred at 87.5% SD 3.3% of vamax, this relationship was interpreted as being an expression of the correlation between vamax and v. Calculation of vamax provided a useful means of analysing the performances. At the level of achievement studied, v sustained over 3000 m corresponded to vamax.(ABSTRACT TRUNCATED AT 250 WORDS)     

Alteration of division polarity and preprophase band orientation in stomatogenesis by light

We have developed an experimental system in which the irradiation with a red light pulse induces stomatal disorientation in the hypocotyl epidermis ofCucumis sativus L. In this system, the orientation of the division plane in guard mother cells was not defined correctly. Preprophase bands formed in these cells but their orientation was abnormal.     

Impact of landscape patterns on ecological vulnerability and ecosystem service values: An empirical analysis of Yancheng Nature Reserve in China

In this study, an ecological vulnerability assessment indicator system was constructed using the “exposure–climate sensitivity–adaptive capacity” framework according to the theory of ecological vulnerability. An improved ecosystem service value calculation model was proposed based on empirical parameters. Using Yancheng Nature Reserve in China as an example, we employed remote sensing data to conduct an empirical analysis of the changes in the spatiotemporal pattern, ecological vulnerability, and ecosystem services of typical landscape types over the period from 1987 to 2013. The statistical results derived from landscape pattern indices (LPIs) showed that during the investigation period, three natural wetland landscape types (i.e., Spartina alterniflora, Suaeda glauca, and Phragmites australis) showed gradually increased fragmentation in the study area. The ecological vulnerability scores of the major landscape types (in descending order) were P. australis (0.053), farmland (0.047), S. alterniflora (0.042), S. glauca (0.031), and bare mud flat (0.002). The results derived from the ecosystem service value fluctuation index (ESVFI) showed that from 1987 to 1997, the value of the ecosystem services provided by S. glauca and P. australis wetlands decreased, whereas that of S. alterniflora wetlands increased continuously. From 2002 to 2013, the value of the ecosystem services provided by all three types of natural wetland showed a downward trend. In conclusion, the combined effects of human and natural factors, including the expansion of farmland and the invasion of S. alterniflora, and changes in seawater erosion and deposition led to changes in the landscape patterns, ecological vulnerability, and ecosystem services of the Yancheng Nature Reserve during the investigation period. The results indicate that a “dual adaptive” management system based on natural succession and supplemented by appropriate manual intervention should be implemented, and the management efficiency and flexibility should be improved to meet the common needs of biodiversity conservation and economic development.     

Asymptotic Relative Efficiency of Some Jackknife Estimators of a Common Odds Ratio

In the situation of several 2 × 2 tables the asymptotic relative efficiencies of certain jackknife estimators of a common odds ratio are investigated in the case that the number of tables is fixed while the sample sizes within each table tend to infinity. The estimators show very good results over a wide range of parameters. Some situations in which the estimators have low asymptotic relative efficiency are pointed out:.     

The facets of olfactory learning

Volatile chemicals in the environment provide ethologically important information to many animals. However, how animals learn to use this information is only beginning to be understood. This review highlights recent experimental advances elucidating olfactory learning in rodents, ranging from adaptations to the environment to task-dependent refinement and multisensory associations. The broad range of phenomena, mechanisms, and brain areas involved demonstrate the complex and multifaceted nature of olfactory learning.